General Cell Based Assays
American Research Products Inc ETV4 Transcription Factor Activity Assay
ETV4 Transcription Factor Activity Assay
Eurofins Discoverx PathHunter eXpress GPR85 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0409E2ACP1L)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HCK Kinase Binding Assay, 2pl Kit. (96-0041E15CP0M)
The InCELL Hunter eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OPRM1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0107E2CP2L)
The cAMP Hunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met-enkephalin, morphine) based activation of OPRM1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRM1, a naturally Gi/Go coupled GPCR. Upon activation of OPRM1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ELABSCIENCE Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method) (E-BC-K109-M) 96 Tests
Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce hydrogen peroxide. In the presence of Trinder's reagent, hydrogen peroxide is catalyzed by peroxidase (POD) to produce a red quinoneimine dye, whose color intensity is directly proportional to the TC concentration and can be measured at 510 nm.
Eurofins Discoverx PathHunter eXpress GPR6 U2OS β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0634E3CP1L)
The PathHunter GPR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR6 and EA-tagged β-Arrestin-2. Activation of GPR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical LDL Uptake Flow Cytometry48tSt
Cayman’s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight™ 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. In addition, multiplexing with other markers, such as LDLR expression, is possible to gain more information from a single experiment. Lovastatin is included in this kit as a control modulator of LDL uptake by hepatocytes. The reagents provided in this kit are sufficient to test 48 samples by FC.
Abcam Maleimide Quantification Assay Kit, 200 tests per kit
Maleimide Quantification Assay Kit, 200 tests per kit
The product is subject to the following: Abcam Restricted Use Statement
Aat Bioquest iFluor™ 350 succinimidyl ester
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Abcam Lipid Assay Kit (neutral lipids)
Lipid Assay Kit (neutral lipids)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CXCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0248E2CP2L)
The PathHunter CXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR7 and EA-tagged β-Arrestin-2. Activation of CXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human MR Nuclear Translocation Cell-Based Assay Kit (CHO-K1)
The PathHunter Human MR Stable Cell Line Nuclear Translocation Assay (CHO-K1) contains a stable clonal cell line used to measure MR nuclear hormone receptor activation via nuclear translocation. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human CXCL13 (CXCR5) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human CXCL13 (CXCR5) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CXCR5 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADGRB2 (BAI2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADGRB2 (BAI2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BAI2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Revvity Health Sciences Inc HTRF Human Pro-Collagen Type 1 Detection Kit, 500 Assay Points
HTRF Human Pro-Collagen Type 1 Detection Kit, 500 Assay Points